l 陽性對照 基因選擇包括 GAPDH, GFP, Luciferase，抑制效率可達 90%
l 陰性對照 修飾選擇：5'-Fluorescein
l 產量 5 nmole, 10 nmole, 20 nmole
l 純化方式 OPC 或 HPLC (螢光修飾建議選擇 HPLC)
包括陽性對照與陰性對照參考序列列表如下
|
種類
|
品項
|
序列 (5'->3')
|
|
Positive Control
陽性對照
|
GAPDH
|
AUUCCAUGGCACCGUCAAG
|
|
Green Fluorescent Prot.
|
GGCUACGUCCAGGAGCGCACC
|
|
Lamin A/C (human)
|
CUGGACUUCCAGAAGAACA
|
|
Lamin B2
|
GAGGAGGAGGAAGCCGAGU
|
|
Luciferase GL2
|
CGUACGCGGAAUACUUCGA
|
|
Luciferase GL3
|
CUUACGCUGAGUACUUCGA
|
|
Nonspecific control
陰性對照
|
31% GC content
|
UAAUGUAUUGGAACGCAUA
|
|
47% GC content
|
AGGUAGUGUAAUCGCCUUG
|
|
68% GC content
|
UGCGCUAGGCCUCGGUUGC
|
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